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Kosgodage, Uchini S., Matewele, Paul, Awamaria, Brigitte, Kraev, Igor, Warde, Purva, Mastroianni, Giulia, Nunn, Alistair V., Guy, Geoffrey W., Bell, Jimmy D., Inal, Jameel and Lange, Sigrun (2019) Cannabidiol Is a novel modulator of bacterial membrane vesicles. Frontiers in Cellular and Infection Microbiology, 9. pp. 1-13. ISSN 2235-2988
Membrane vesicles (MVs) released from bacteria participate in cell communication and host-pathogen interactions. Roles for MVs in antibiotic resistance are gaining increased attention and in this study we investigated if known anti-bacterial effects of cannabidiol (CBD), a phytocannabinoid from Cannabis sativa, could be in part attributed to effects on bacterial MV profile and MV release. We found that CBD is a strong inhibitor of MV release from Gram-negative bacteria (E. coli VCS257), while inhibitory effect on MV release from Gram-positive bacteria (S. aureus subsp. aureus Rosenbach) was negligible. When used in combination with selected antibiotics, CBD significantly increased the bactericidal action of several antibiotics in the Gram-negative bacteria. In addition, CBD increased antibiotic effects of kanamycin in the Gram-positive bacteria, without affecting MV release. CBD furthermore changed protein profiles of MVs released from E. coli after 1 h CBD treatment. Our findings indicate that CBD may pose as a putative adjuvant agent for tailored co-application with selected antibiotics, depending on bacterial species, to increase antibiotic activity, including via MV inhibition, and help reduce antibiotic resistance.
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