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Ahmad Zahra, Maftuha, Tasnim Toma, Tasfia, Nasreen, Shamima, Zarin, Zarin Tasnim Rafia, Khan, Zerin Tasnim Siddiqa Elma and Haque, Fahim Kabir Monjurul (2025) Characterizing the co-existence of metallo-β-lactamase-producing and extended spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolates in community wastewater samples of Dhaka, Bangladesh. Journal of Water and Health. ISSN 1996-7829
Escherichia coli and Klebsiella pneumoniae isolates with multiple antibiotic-resistant genes in wastewater poses serious public health risks, as they can potentially contaminate the food and water supply. The main aim of this study was to isolate and identify E. coli and K. pneumoniae from community wastewater samples, determine their antibiotic resistance profiles, and their antibiotic-resistant genes. From the northern part of Dhaka, Bangladesh, 36 wastewater samples were collected across 11 different areas, which were then serially diluted, and cultured using selective media. Isolates were identified via polymerase chain reaction. Out of the 197 isolates identified, E. coli and K. pneumoniae accounted for 55.8% (n = 110) and 44.2% (n = 87), respectively. Antibiotic susceptibility tests revealed multidrug resistance (MDR) in 30% of E. coli and 35.56% of K. pneumoniae isolates. Among E. coli, the prevalence of antibiotic-resistant genes included blaNDM-1 (8.9%), blaSHV (13.9%), and blaCTX-M (7.6%). In K. pneumoniae, the percentages were blaNDM-1 (12.8%), blaSHV (4.3%), and blaCTX-M (5.0%). Co-existence of multiple antibiotic-resistant genes was observed in 4.54% of E. coli isolates (n = 5) and 5.74% of K. pneumoniae isolates (n = 5). This suggests the escalating issue of infectious species becoming increasingly resistant to antibiotics in wastewater systems.
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