Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy

De Sousa, Karina P., Rossi, Izadora, Abdullahi, Mahamed, Ramirez, Marcel Ivan, Stratton, Dan and Inal, Jameel (2022) Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy. Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology, 15(1) (e1835). pp. 1-29. ISSN 1939-0041

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Abstract / Description

Extracellular vesicles (EVs) are a unique and heterogeneous class of lipid bilayer nanoparticles secreted by most cells. EVs are regarded as important mediators of intercellular communication in both prokaryotic and eukaryotic cells due to their ability to transfer proteins, lipids and nucleic acids to recipient cells. In addition to their physiological role, EVs are recognized as modulators in pathological processes such as cancer, infectious diseases, and neurodegenerative disorders, providing new potential targets for diagnosis and therapeutic intervention. For a complete understanding of EVs as a universal cellular biological system and its translational applications, optimal techniques for their isolation and characterization are required. Here, we review recent progress in those techniques, from isolation methods to characterization techniques. With interest in therapeutic applications of EVs growing, we address fundamental points of EV-related cell biology, such as cellular uptake mechanisms and their biodistribution in tissues as well as challenges to their application as drug carriers or biomarkers for less invasive diagnosis or as immunogens.

Item Type:	Article
Additional Information:	This is the peer reviewed version of the article, which has been published in final form at https://doi.org/10.1002/wnan.1835. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
Uncontrolled Keywords:	isolation methods; extracellular vesicles; EV characterization; EV therapeutics; EV analysis
Subjects:	600 Technology > 610 Medicine & health
Department:	School of Human Sciences
SWORD Depositor:	Pub Router
Depositing User:	Pub Router
Date Deposited:	12 Aug 2022 15:01
Last Modified:	20 Feb 2023 12:09
URI:	https://repository.londonmet.ac.uk/id/eprint/7852

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