Epidithiodiketopiperazines inhibit protein degradation by targeting Proteasome Deubiquitinase Rpn11

Li, Jing, Zhang, Yaru, Sil dos Santos, Bruno, Wang, Feng, Ma, Yuyong, Perez, Christian, Yang, Yanling, Peng, Junmin, Cohen, Seth M., Chou, Tsui-Fen, Hilton, Stephen T. and Deshaies, Raymond J. (2018) Epidithiodiketopiperazines inhibit protein degradation by targeting Proteasome Deubiquitinase Rpn11. Cell chemical biology. ISSN 2451-9448

1-s2.0-S2451945618302642-main.pdf - Accepted Version

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Abstract / Description

The 26S proteasome is the major proteolytic machine for breaking down cytosolic and nuclear proteins in eukaryotes. Due to the lack of a suitable assay, it is difficult to measure routinely and quantitatively the breakdown of proteins by the 26S proteasome in vitro. In the present study, we developed an assay to monitor proteasome-mediated protein degradation. Using this assay, we discovered that epidithiodiketopiperazine (ETPs) blocked the degradation of our model substrate in vitro. Further characterization revealed that ETPs inhibited proteasome function by targeting the essential proteasomal deubiquitinase Rpn11 (POH1/PSMD14). ETPs also inhibited other JAMM (JAB1/MPN/Mov34 metalloenzyme) proteases such as Csn5 and AMSH. An improved ETP with fewer non-specific effects, SOP11, stabilized a subset of proteasome substrates in cells, induced the unfolded protein response, and led to cell death. SOP11 represents a class of Rpn11 inhibitor and provides an alternative route to develop proteasome inhibitors. [Abstract copyright: Copyright © 2018 Elsevier Ltd. All rights reserved.]

Item Type: Article
Additional Information: ** From PubMed via Jisc Publications Router. ** History: received 15-02-2018; revised 23-05-2018; accepted 25-07-2018.
Uncontrolled Keywords: Capzimin, JAMM protease, POH1, PSMD14, Rpn11, epidithiodiketopiperazine, gliotoxin, proteasome, protein degradation, ubiquitin
Subjects: 500 Natural Sciences and Mathematics > 570 Life sciences; biology
Department: School of Human Sciences
SWORD Depositor: Pub Router
Depositing User: Pub Router
Date Deposited: 14 Sep 2018 10:24
Last Modified: 09 Aug 2019 11:48
URI: https://repository.londonmet.ac.uk/id/eprint/3037


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