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Arter, Eliz (2025) Anti-cancerous and anti-metastatic effects of Corchorus Olitorius on breast cancer cell lines. Doctoral thesis, London Metropolitian University.
Breast cancer is one of the leading conditions affecting women worldwide, with a high incidence and mortality rate, including Cyprus. Several factors contribute to the development of breast cancer. These include genetic predisposition, reproductive health and modifiable factors such as dietary habits, physical activity, smoking and alcohol consumption.
Given that diet is a significant determinant in the development of breast cancer, the present study focused on Corchorus olitorius (commonly known as jute), a locally grown and widely consumed dark green leafy vegetable. The investigation aimed to explore its potential anti-cancer effects on selected breast cancer cell lines: MCF-7, MDA-MB-231 and MDA-MB-468. Acetone, hexane and methanol were used to extract bioactive compounds from C. olitorius leaves based on their solubility.
These extracts were subsequently tested to evaluate their potential anti-proliferative, anti-adhesive, anti-migratory and anti-invasive effects in vitro. The findings suggest that MDA-MB-468 cells were the most affected in proliferation, adhesion and migration assays, with the hexane and methanol extracts being more potent in possessing anti-cancer effects. These could be attributed to the major compounds identified in GC-MS analysis which include α-linolenic acid and palmitic acid.
Total phenolic and flavonoid content assays in the present study confirmed the presence of polyphenolic compounds in all C. olitorius leaf extracts. The DPPH radical scavenging assay has shown that the methanol has the lowest IC50 value (51.70 μg/mL), showing its higher antioxidant capacity.
Another objective was to investigate the overall dietary habits of adult women in Northern Cyprus through a survey and to draw potential parallels between the survey findings and the in vitro experiments. Even though the survey study was planned as a pilot study with a limited number of participants, certain eating and cooking habits were observable, such as the lower omega 3:omega 6 fatty acid ratio in the intakes, rare use of steaming and the predominant use of olive oil as the staple oil in the region.
The significance of the present study lies in the preliminary evidence it provides regarding the collective anti-cancer effects of C. olitorius extracts on breast cancer cell lines. These findings could serve as a foundation for future research
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