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Sisa, Claudia, Kholia, Sharad, Naylor, Jordan, Herrera Sanchez, Maria Beatriz, Bruno, Stefania, Deregibus, Maria Chiara, Camussi, Giovanni, Inal, Jameel M., Lange, Sigrun and Hristova, Mariya (2019) Mesenchymal stromal cell derived extracellular vesicles reduce hypoxia-ischaemia induced perinatal brain injury. Frontiers in physiology, 10 (282). pp. 1-10. ISSN 1664-042X
BACKGROUND
Neonatal hypoxic-ischemic (HI) insult is a leading cause of disability and death in newborns, with therapeutic hypothermia being the only currently available clinical intervention. Thus there is a great need for adjunct and novel treatments for enhanced or alternative post-HI neuroprotection. Extracellular vesicles (EVs) derived from mesenchymal stromal/stem cells (MSCs) have recently been shown to exhibit regenerative effects in various injury models. Here we present findings showing neuroprotective effects of MSC-derived EVs in the Rice-Vannucci model of severe HI-induced neonatal brain insult.
METHODS
Mesenchymal stromal/stem cell-derived EVs were applied intranasally immediately post HI-insult and behavioral outcomes were observed 48 h following MSC-EV treatment, as assessed by negative geotaxis. Brains were thereafter excised and assessed for changes in glial responses, cell death, and neuronal loss as markers of damage at 48 h post HI-insult.
RESULTS
Brains of the MSC-EV treated group showed a significant decrease in microglial activation, cell death, and percentage tissue volume loss in multiple brain regions, compared to the control-treated groups. Furthermore, negative geotaxis test showed improved behavioral outcomes at 48 h following MSC-EV treatment.
CONCLUSION
Our findings highlight the clinical potential of using MSC-derived EVs following neonatal hypoxia-ischaemia.
Available under License Creative Commons Attribution 4.0.
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