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Jorfi, Samireh, Ansa-Addo, Ephraim Abrokwa, Mariniello, Katia, Warde, Purva, Bin Senian, Ahmad Asyraf, Stratton, Dan, Bax, Bridget E., Levene, Michelle, Lange, Sigrun and Inal, Jameel Malhador (2023) A Coxsackievirus B1-mediated nonlytic Extracellular Vesicle-to-cell mechanism of virus transmission and its possible control through modulation of EV release. The Journal of general virology, 104 (9). pp. 1-41. ISSN 1465-2099
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Abstract / Description
Like most non-enveloped viruses, CVB1 mainly uses cell lysis to spread. Details of a nonlytic virus transmission remain unclear. Extracellular Vesicles (EVs) transfer biomolecules between cells. We show that CVB1 entry into HeLa cells results in apoptosis and release of CVB1-induced 'medium-sized' EVs (CVB1-mEVs). These mEVs (100-300 nm) harbour CVB1 as shown by immunoblotting with anti-CVB1-antibody; viral capsids were detected by transmission electron microscopy and RT-PCR revealed CVB1 RNA. The percentage of mEVs released from CVB1-infected HeLa cells harbouring virus was estimated from TEM at 34 %. Inhibition of CVB1-mEV production, with calpeptin or siRNA knockdown of in HeLa cells limited spread of CVB1 suggesting these vesicles disseminate CVB1 virions to new host cells by a nonlytic EV-to-cell mechanism. This was confirmed by detecting CVB1 virions inside HeLa cells after co-culture with CVB1-mEVs; EV release may also prevent apoptosis of infected cells whilst spreading apoptosis to secondary sites of infection.
| Item Type: | Article |
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| Additional Information: | © Samireh Jorfi, Ephraim Abrokwa Ansa-Addo, Katia Mariniello, Purva Warde, Ahmad Asyraf bin Senian, Dan Stratton, Bridget E. Bax, Michelle Levene, Sigrun Lange, Jameel Malhador Inal, 2023. The definitive peer reviewed, edited version of this article is published in Microbiology Society, volume 104, number 9, 2023, DOI: https://doi.org/10.1099/jgv.0.001884 |
| Uncontrolled Keywords: | Coxsackievirus B1; Extracellular Vesicles; Nonlytic transmission |
| Subjects: | 500 Natural Sciences and Mathematics > 570 Life sciences; biology |
| Department: | School of Human Sciences |
| Depositing User: | Jameel Inal |
| Date Deposited: | 06 Sep 2023 15:04 |
| Last Modified: | 26 Sep 2023 13:34 |
| URI: | https://repository.londonmet.ac.uk/id/eprint/8748 |
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