Cross-serotype immunity induced by immunization with a conserved rhinovirus capsid protein

Glanville, Nicholas and McLean, Gary R. and Guy, Bruno and Lecouturier, Valerie and Berry, Catherine and Girerd, Yves and Gregoire, Christophe and Walton, Ross P. and Pearson, Rebecca M. and Kebadze, Tatiana and Burdin, Nicolas and Bartlett, Nathan W. and Almond, Jeffrey W. and Johnston, Sebastian L. (2013) Cross-serotype immunity induced by immunization with a conserved rhinovirus capsid protein. PLoS pathogens, 9 (9). e1003669. ISSN 1553-7374

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Abstract

Human rhinovirus (RV) infections are the principle cause of common colds and precipitate asthma and COPD exacerbations. There is currently no RV vaccine, largely due to the existence of ∼150 strains. We aimed to define highly conserved areas of the RV proteome and test their usefulness as candidate antigens for a broadly cross-reactive vaccine, using a mouse infection model. Regions of the VP0 (VP4+VP2) capsid protein were identified as having high homology across RVs. Immunization with a recombinant VP0 combined with a Th1 promoting adjuvant induced systemic, antigen specific, cross-serotype, cellular and humoral immune responses. Similar cross-reactive responses were observed in the lungs of immunized mice after infection with heterologous RV strains. Immunization enhanced the generation of heterosubtypic neutralizing antibodies and lung memory T cells, and caused more rapid virus clearance. Conserved domains of the RV capsid therefore induce cross-reactive immune responses and represent candidates for a subunit RV vaccine.

Item Type: Article
Uncontrolled Keywords: human rhinovirus infections, human RV infections, rhinoviruses
Subjects: 600 Technology > 610 Medicine & health
Department: School of Human Sciences
Depositing User: Gary Mclean
Date Deposited: 03 Aug 2015 09:12
Last Modified: 18 Aug 2015 10:32
URI: http://repository.londonmet.ac.uk/id/eprint/729

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