Stimulation of Interleukin-2 [IL-2] Release by Rhizophora mangle Bark Aqueous Extracts and Its Fractions

de Armas, Elizabeth and Escobar, Arturo and Faure, Roberto and Marrero, Evangelina and Bligh, Annie S. W. and Branford-White, Christopher J. and White, Kenneth (2016) Stimulation of Interleukin-2 [IL-2] Release by Rhizophora mangle Bark Aqueous Extracts and Its Fractions. European Journal of Medicinal Plants, 15 (2). pp. 1-10. ISSN 2331-0894

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Abstract

Aims: The objective of the present study was to prepare fractions of polyphenols based on their ability to stimulate release of interleukin-2 (IL-2) from a human T-cell line, Jurkat, in the presence or absence of phorbol myristic acetate (PMA), and to identify the candidate components responsible for this activity.
Study Design: Rhizophora mangle L. [Rhizophoraceae] bark was collected in occident zone of CUBA, was boiled in distilled water and freezer dried for its fractionation.
Methodology: The IL2-releasing the activity of different fractions were quantified using a BDOptEIA Human IL-2 ELISA kit using Jurkat Cells and PMA during the test. The ESI–MS fingerprints of the extracts [ESI-MS/MS] were acquired by the negative ion mode using a Micromass-Waters QTOF mass spectrometer.
Results: Guided fractionations from Rhizophora mangle bark aqueous extracts in the evaluation of releasing activity of interleukin 2-stimulated and unstimulated with Jurkat T cells in the presence of PMA showed that the butanolic fraction had an interleukin 2 production of 250 pg mL-1 and an 89.9% yield of procyanidins. Mass spectral studies of the butanolic fraction reflected the presence of compounds that varied between 1000-1333 m/z, indicating the presence of procyanidins up to a tetramer polymerization level linked to glycosides based on monomeric units [epicatechin / catechin]. An increase of IL-2, without prior stimulation with PMA, in the Jurkat T cell model, had not been previously reported for the phenolic compounds.
Conclusion: The compounds characterized preliminarily confirmed the structural diversity of polyphenols present in Rhizophora mangle L plant and its capacity to stimulate release of IL 2.

Item Type: Article
Uncontrolled Keywords: Rhizophora mangle L, interleukin 2, procyanidins, Jurkat T-cell
Subjects: 500 Natural Sciences and Mathemetics > 570 Life sciences; biology
Department: School of Human Sciences
Depositing User: Kenneth White
Date Deposited: 28 Jun 2016 09:25
Last Modified: 28 Jun 2016 09:25
URI: http://repository.londonmet.ac.uk/id/eprint/641

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