GLP-2 decreases food intake in the dorsomedial hypothalamic nucleus (DMH) through Exendin (9-39) in male Sprague-Dawley (SD) rats

Sun, Huiling, Meng, Kai, Hou, Lin, Shang, Lijun and Yan, Jianqun (2021) GLP-2 decreases food intake in the dorsomedial hypothalamic nucleus (DMH) through Exendin (9-39) in male Sprague-Dawley (SD) rats. Physiology and Behavior, 229 (113253). ISSN 0031-9384

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Official URL: https://doi.org/10.1016/j.physbeh.2020.113253

Abstract / Description

Glucagon-like peptide 2 (GLP-2), a member of Glucagon peptide family involved in regulating energy metabolism, can be produced and secreted by preproglucagonergic (PPG) neurons in the brain. GLP-2 reduces food intake but at which brain sites GLP-2 exerts its feeding-suppress effects are still unclear. In this study, we used the stereological microinjection technique and behavioral test to examine the functions of locally delivered GLP-2 into DMH on feeding behavior. We compared effects of different concentration of GLP-2 on the food intake behavior in free-feeding rats and fasted-refeeding rats. We found that GLP-2 inhibited food intake in fasted rats after a short-term intervention in a dose-dependent manner. Importantly, the effects of locally delivered GLP-2 can be blocked by specific GLP-1 receptor antagonist Exendin(9-39), but not the melanocortin-4 receptor antagonist SHU9119, indicating the involvement of specificity of GLP-2 signaling in regulating the feeding behavior. Taken together, our data revealed that GLP-2 peptide pharmacologically inhibited food intake in DMH and this effect could be blocked functionally by Exendin(9-39).

Item Type: Article
Uncontrolled Keywords: Microinjection; Glucagon-like peptide 2 (GLP-2); the dorsomedial hypothalamic nucleus (DMH); Sprague-Dawley (SD) rats; Stereotaxic surgery
Subjects: 500 Natural Sciences and Mathematics > 570 Life sciences; biology
500 Natural Sciences and Mathematics > 590 Animals (Zoology)
Department: School of Human Sciences
Depositing User: Lijun Shang
Date Deposited: 24 Nov 2020 13:10
Last Modified: 08 Jan 2021 09:30
URI: http://repository.londonmet.ac.uk/id/eprint/6189

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