Optimization of enzymatic hydrolysis conditions of Caspian kutum (Rutilusfrisiikutum) by-product for production of bioactive Peptides with antioxidative properties

Azizi Khesal, Mona, Sharifan, Anousheh, Hoseini, Ebrahim and Ghavami, Abdollah (2019) Optimization of enzymatic hydrolysis conditions of Caspian kutum (Rutilusfrisiikutum) by-product for production of bioactive Peptides with antioxidative properties. International Journal of Peptide Research and Therapeutics. ISSN 1573-3149

[img]
Preview
Text
Optimization-of-enzymatic-hydrolysis-conditions-of-Caspian-kutum.pdf - Accepted Version
Available under License Creative Commons Attribution Non-commercial No Derivatives 4.0.

Download (833kB) | Preview

Abstract / Description

The enzymatic hydrolysis was performed by Alcalase to recover the fish protein hydrolysate from Caspian kutum by-product (CB). The degree of hydrolysis (DH) was applied for monitoring the hydrolysis reaction of CB. The response surface methodology was applied based on a D-optimal design to perform the optimization process for obtaining the high yield of CB protein hydrolysate. The effect of four independent variables including pH (7.5–8.5), temperature (45–55 °C), time (1–3 h), and enzyme concentration (0.5–1.5% w/w) on DH was studied. The results indicated that the predicted and actual values of the optimum condition had no significant difference. The optimum enzymatic hydrolysis conditions were achieved at pH 8.5, temperature of 55 °C, enzyme concentration of 1.5% w/w, and time of 3 h, which resulted in the maximum value of DH (19.08%). Antioxidant assays including DPPH scavenging and metal chelating activities showed that Caspian kutum protein hydrolysates had antioxidant properties.

Item Type: Article
Uncontrolled Keywords: fish protein hydrolysate; by-product; optimization; alcalase; hydrolysis; antioxidant
Subjects: 500 Natural Sciences and Mathematics > 570 Life sciences; biology
Department: School of Human Sciences
Depositing User: Abdollah Ghavami
Date Deposited: 13 May 2020 13:29
Last Modified: 13 May 2020 14:14
URI: http://repository.londonmet.ac.uk/id/eprint/5757

Downloads

Downloads per month over past year



Downloads each year

Actions (login required)

View Item View Item