Lists
Lists
Azizi Khesal, Mona, Sharifan, Anousheh, Hoseini, Ebrahim and Ghavami, Abdollah (2019) Optimization of enzymatic hydrolysis conditions of Caspian kutum (Rutilusfrisiikutum) by-product for production of bioactive Peptides with antioxidative properties. International Journal of Peptide Research and Therapeutics. ISSN 1573-3149
|
Text
Optimization-of-enzymatic-hydrolysis-conditions-of-Caspian-kutum.pdf - Accepted Version Available under License Creative Commons Attribution Non-commercial No Derivatives 4.0. Download (833kB) | Preview |
Abstract / Description
The enzymatic hydrolysis was performed by Alcalase to recover the fish protein hydrolysate from Caspian kutum by-product (CB). The degree of hydrolysis (DH) was applied for monitoring the hydrolysis reaction of CB. The response surface methodology was applied based on a D-optimal design to perform the optimization process for obtaining the high yield of CB protein hydrolysate. The effect of four independent variables including pH (7.5–8.5), temperature (45–55 °C), time (1–3 h), and enzyme concentration (0.5–1.5% w/w) on DH was studied. The results indicated that the predicted and actual values of the optimum condition had no significant difference. The optimum enzymatic hydrolysis conditions were achieved at pH 8.5, temperature of 55 °C, enzyme concentration of 1.5% w/w, and time of 3 h, which resulted in the maximum value of DH (19.08%). Antioxidant assays including DPPH scavenging and metal chelating activities showed that Caspian kutum protein hydrolysates had antioxidant properties.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | fish protein hydrolysate; by-product; optimization; alcalase; hydrolysis; antioxidant |
| Subjects: | 500 Natural Sciences and Mathematics > 570 Life sciences; biology |
| Department: | School of Human Sciences |
| Depositing User: | Abdollah Ghavami |
| Date Deposited: | 13 May 2020 13:29 |
| Last Modified: | 13 May 2020 14:14 |
| URI: | https://repository.londonmet.ac.uk/id/eprint/5757 |
Downloads
Downloads per month over past year
Downloads each year
Actions (login required)
 |
View Item |