The expression of CD47 and its association with 2,3-DPG levels in stored leuco-reduced blood units

Eldesouky, Nermeen A., Abo El Fetouh, Randa M., Hafez, Ahmed Abdel, Gad, Alaa and Kamal, Maha M. (2019) The expression of CD47 and its association with 2,3-DPG levels in stored leuco-reduced blood units. Transfusion clinique et biologique : journal de la Société française de transfusion sanguine, 26 (4). pp. 279-283. ISSN 1953-8022

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Abstract / Description

Red blood cell (RBC) aging in transfusion medicine is characterized by alteration of many biochemical and morphological integrity of the cell referred to as red cell storage lesion (RCSL), CD47 is a protective marker expressed on RBCs that salvage the cell from phagocytosis. 2,3-diphosphoglycerate (2,3-DPG) tends to have a greater affinity towards deoxygenated hemoglobin. Any oxygen unloading at tissue capillaries are facilitated by 2,3-DPG, and any alterations in its levels can significantly interfere with oxygen release. Alteration of both CD47 expression and 2,3-DPG levels during red cell storage may serve as markers in the development of RCSL. The aim of this study was to validate the impact of storage time and leuco-depletion on CD47 expression on the RBCs, which could be a prospective marker for detection of RBCs viability and to clarify if the changes in CD47 expression and 2,3-DPG levels are correlated during storage of Packed RBCs. One hundred samples from Packed RBCs units were divided into two groups [Group 1 comprised unfiltered packed red cell units (n=50), whereas Group 2 included filtered "leuco-reduced" red cell units (n=50)]. Collection of samples was executed on days 0, 1 and 21. Each sample was measured for 2,3-DPG and alteration of CD47 expression on RBC using flow cytometry. Decreased CD47 expression along the storage period was statistically significant in both groups (P<0.05). Interestingly, the expression of CD47 was significantly higher in group 2 than group 1 on day zero, 1st and 21st days (P<0.05). Additionally, a statistically significant decrease in 2,3-DPG level was detected at day 21 of storage in group 1 compared to group 2 with a P-value of <0.001. There was a significant positive correlation (r=0.570, P<0.001) between CD47 MFI on RBC during storage and the level of 2,3-DPG at day 21 from packed RBCs storage. Older unfiltered RBC possesses lower expression of CD47 and low levels of 2,3-DPG, however filtration (leucoreduction) of RBCs units may help to retain considerable levels of 2,3-DPG and CD47 and hence sustains preservation of RBCs through reduction of phagocytosis.

Item Type:	Article
Additional Information:	** From PubMed via Jisc Publications Router [Abstract copyright: Copyright © 2019 Elsevier Masson SAS. All rights reserved.]
Uncontrolled Keywords:	2,3-DPG, CD47, Cytométrie en flux, Flow cytometry, Leuco-depletion, Leucodéplétion, RCSL
Subjects:	500 Natural Sciences and Mathematics > 570 Life sciences; biology 600 Technology > 610 Medicine & health
Department:	School of Human Sciences
SWORD Depositor:	Pub Router
Depositing User:	Pub Router
Date Deposited:	13 Mar 2019 10:34
Last Modified:	21 Apr 2020 11:54
URI:	https://repository.londonmet.ac.uk/id/eprint/4626

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