Cannabidiol affects extracellular vesicle release, miR21 and miR126, and reduces prohibitin protein in glioblastoma multiforme cells

Kosgodage, Uchini S., Uysal-Onganer, Pinar, MacLatchy, Amy, Mould, Rhys, Nunn, Alistair V., Guy, Geoffrey W., Kraev, Igor, Chatterton, Nicholas P., Thomas, E. Louise, Inal, Jameel M., Bell, Jimmy D. and Lange, Sigrun (2018) Cannabidiol affects extracellular vesicle release, miR21 and miR126, and reduces prohibitin protein in glioblastoma multiforme cells. Translational oncology, 12 (3). pp. 513-522. ISSN 1936-5233

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Abstract / Description

Glioblastoma multiforme (GBM) is the most common and aggressive form of primary malignant brain tumor in adults, with poor prognosis. Extracellular vesicles (EVs) are key-mediators for cellular communication through transfer of proteins and genetic material. Cancers, such as GBM, use EV release for drug-efflux, pro-oncogenic signaling, invasion and immunosuppression; thus the modulation of EV release and cargo is of considerable clinical relevance. As EV-inhibitors have been shown to increase sensitivity of cancer cells to chemotherapy, and we recently showed that cannabidiol (CBD) is such an EV-modulator, we investigated whether CBD affects EV profile in GBM cells in the presence and absence of temozolomide (TMZ). Compared to controls, CBD-treated cells released EVs containing lower levels of pro-oncogenic miR21 and increased levels of anti-oncogenic miR126; these effects were greater than with TMZ alone. In addition, prohibitin (PHB), a multifunctional protein with mitochondrial protective properties and chemoresistant functions, was reduced in GBM cells following 1 h CBD treatment. This data suggests that CBD may, via modulation of EVs and PHB, act as an adjunct to enhance treatment efficacy in GBM, supporting evidence for efficacy of cannabinoids in GBM.

Item Type: Article
Additional Information: ** From Europe PMC via Jisc Publications Router.
Uncontrolled Keywords: Glioblastoma multiforme (GBM); malignant brain tumours; cancers; extracellular vesicles (EVs); temozolomide (TMZ)
Subjects: 500 Natural Sciences and Mathematics > 570 Life sciences; biology
600 Technology > 610 Medicine & health
Department: School of Human Sciences
SWORD Depositor: Pub Router
Depositing User: Pub Router
Date Deposited: 13 Mar 2019 15:53
Last Modified: 13 Mar 2019 15:53
URI: http://repository.londonmet.ac.uk/id/eprint/4575

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