A novel and rapid method for obtaining high titre intact prion strains from mammalian brain

Wenborn, Adam, Terry, Cassandra, Gros, Nathalie, Joiner, Susan, D’Castro, Laura, Panico, Silvia, Sells, Jessica, Cronier, Sabrina, Linehan, Jacqueline M., Brandner, Sebastian, Saibil, Helen R., Collinge, John and Wadsworth, Jonathan D. F. (2015) A novel and rapid method for obtaining high titre intact prion strains from mammalian brain. Scientific Reports, 5 (1). ISSN 2045-2322

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Official URL: http://dx.doi.org/10.1038/srep10062

Abstract / Description

Mammalian prions exist as multiple strains which produce characteristic and highly reproducible phenotypes in defined hosts. How this strain diversity is encoded by a protein-only agent remains one of the most interesting and challenging questions in biology with wide relevance to understanding other diseases involving the aggregation or polymerisation of misfolded host proteins. Progress in understanding mammalian prion strains has however been severely limited by the complexity and variability of the methods used for their isolation from infected tissue and no high resolution structures have yet been reported. Using high-throughput cell-based prion bioassay to re-examine prion purification from first principles we now report the isolation of prion strains to exceptional levels of purity from small quantities of infected brain and demonstrate faithful retention of biological and biochemical strain properties. The method's effectiveness and simplicity should facilitate its wide application and expedite structural studies of prions.

Item Type: Article
Uncontrolled Keywords: prion, prion disease, prion protein, prion structure, electron tomography
Subjects: 500 Natural Sciences and Mathematics > 570 Life sciences; biology
Department: School of Human Sciences
Depositing User: Cassandra Terry
Date Deposited: 19 Nov 2018 10:46
Last Modified: 19 Nov 2018 10:46
URI: http://repository.londonmet.ac.uk/id/eprint/3843

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