Microvesicles released from Giardia intestinalis disturb host-pathogen response in vitro

Evans-Osses, Ingrid and Mojoli, Andres and Monguió-Tortajada, Marta and Marcilla, Antonio and Aran, Veronica and Amorim, Maria and Inal, Jameel and Borràs, Francesc E. and Ramirez, Marcel I. (2017) Microvesicles released from Giardia intestinalis disturb host-pathogen response in vitro. European Journal of Cell Biology. ISSN 0171-9335

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Abstract

Giardia intestinalis (G.I), is an anaerobic protozoan and the aetiological agent of giardiasis, a diarrhoea present worldwide and associated with poverty. G.I has a simple life cycle alternating between cyst and trophozoite. Cysts are transmitted orally to the stomach and transform to trophozoites in the intestine by a multifactorial process. Recently, microvesicles (MVs) have been found to be released from a wide range of eukaryotic cells. We have observed a release of MVs during the life cycle of G.I., identifying MVs from active trophozoites and from trophozoites differentiating to the cyst form. The aim of the current work was to investigate the role of MVs from G.I in the pathogenesis of giardiasis. MVs from log phase were able to increase the attachment of G. intestinalis trophozoites to Caco-2 cells. Moreover, MVs from G. intestinalis could be captured by human immature dendritic cells, resulting in increased activation and allostimulation of human dendritic cells. Lipid rafts participate in the MV biogenesis and in the attachment to Caco-2 cells. Nevertheless, proteomic analysis from two types of MVs has shown slight differences at the protein levels. An understanding of biogenesis and content of MVs derived from trophozoites might have important implications in the pathogenesis of the disease.

Item Type: Article
Uncontrolled Keywords: Microvesicles; Parasite-host cell interactions; Innate immunity; Diarrhoea; Giardia intestinalis; Extracellular vesicle
Subjects: 500 Natural Sciences and Mathemetics > 570 Life sciences; biology
600 Technology > 610 Medicine & health
Department: School of Human Sciences
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Depositing User: Jameel Inal
Date Deposited: 28 Feb 2017 09:12
Last Modified: 28 Feb 2017 09:12
URI: http://repository.londonmet.ac.uk/id/eprint/1204

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